| 20nine | Pennsylvania |
| 20North | |
| 20Twenty Design | |
| 21-6 Productions, Inc. | Colorado |
| 2112 Incubator Chicago | Illinois |
| 212 Creative, LLC | |
| 2140 Interactive, Inc. | Pennsylvania |
| 215 Marketing | |
| 215Secure, LLC | Pennsylvania |
| 216digital | Ohio |
| 21st Century Distributing, Inc. | North Carolina |
| 21st Century Robotics, Inc. | Georgia |
| 21st Century Software, Inc. | Pennsylvania |
| 21st Century Technologies, Inc. | Colorado |
| 22 Miles, Inc. | California |
| 22 Pixels, Inc. | |
| 222 Digital Marketing | |
| 223 Agency | |
| 22nd Century Tech, Inc. | Florida |
| 22nd Software Solutions | New Jersey |
| 22Solutions, LLC | Texas |
| 22Squared | |
| 231 Web Development | |
| 232 Creative | |
| 23andMe, Inc. | California |
| 23blocks, Inc. | Florida |
| 24 Capital | Florida |
| 24 Communications | Alabama |
| 24 Hour Company | Virginia |
| 24 Hour Marketing | |
| 24/7 Customer, Inc. | California |
| 24/7 Education, Inc. | New York |
| 24/7 Logo Services | |
| 24/7 NY Web Design, Inc. | New York |
| 24/7 Techies, LLC | |
| 2424 Studios | Pennsylvania |
| 2440 Media, Inc. | Pennsylvania |
| 247 Digital Marketing | |
| 247, Inc. | California |
| 247Solar | |
| 24By7Security, Inc. | |
| 24hourtek | California |
| 24hrTechSupport | Florida |
| 24M Technologies, Inc. | Massachusetts |
| 24Seven Commerce | California |
| 24Shells, Inc. | New Jersey |
| 24Webstudio | |
| 25madison | New York |
| 260 Web Designs | |
| 2600Hz | Nevada |