| Big 5 distribution | Gauteng |
| Big Fish Development | Eastern Cape |
| BIG Media | Gauteng |
| Big Plan | KwaZulu-Natal |
| Bika Open Source LIMS | Western Cape |
| BiomatrixSolutions | KwaZulu-Natal |
| BioTech Africa | Gauteng |
| Biotrace Software | Free State |
| Bisnet IT & Software Solutions | Eastern Cape |
| BitCo | Gauteng |
| Bitcube | Free State |
| Bite Web Solutions (Pty) Ltd | |
| BitFund | |
| Bits & Bytes CC | Gauteng |
| BiztechAfrica | Gauteng |
| Black Fig Jam (Pty) Ltd | Gauteng |
| Black Snow Communications | Gauteng |
| Black Snow Digital | |
| Blackalsatian | Eastern Cape |
| Blacklight | Gauteng |
| Bladesight | |
| Blank Media | Gauteng |
| Blaze Web Studio | KwaZulu-Natal |
| Blockchain Africa Conference | |
| Blu Flamingo | Gauteng |
| Blue Box | Western Cape |
| Blue Cabin SEO Company | |
| Blue Chip Computers | KwaZulu-Natal |
| Blue Forest Productions | |
| Blue Label Telecoms Ltd | Gauteng |
| Blue Stallion Technologies CC | Gauteng |
| Bluebird | Western Cape |
| Bluegrass Digital | Western Cape |
| Bluejeans IT Solutions | Gauteng |
| BlueLight Webdesign | |
| BlueMagnet | Gauteng |
| BlueSteam | Western Cape |
| Blugecko | Gauteng |
| BluWave Software | Gauteng |
| BNRY Digital | Western Cape |
| BOHN management systems | Western Cape |
| Booksure | Western Cape |
| Borake Netserve (Pty) Ltd | Northern Cape |
| Bounce Design | Western Cape |
| Brainstorm Tech Solutions | |
| Braintree | Gauteng |
| Brandbook | Gauteng |
| BrandSchool | Gauteng |
| BrandsEye | Western Cape |
| Brandsplash (Pty) Ltd | |