| Spheres | Quintana Roo |
| SpicyMinds | Mexico City |
| SpinDigital | Puebla |
| Spiral Media Labs | Sinaloa |
| Sr. Pago | Mexico City |
| Start Up Solutions | Mexico City |
| Startcode | |
| Startup Baja | Baja California |
| Startup Mexico | Mexico City |
| Startup Studio Monterrey, S.A.P.I. de C.V. | Nuevo León |
| StartupGDL | Jalisco |
| StartupLab MX | San Luis Potosí |
| STC | Mexico City |
| Stendhal Pharma | |
| StockWare S.A. de C.V. | Mexico City |
| Stori | Mexico City |
| STX, Soluciones en Línea | Jalisco |
| Style One | Baja California Sur |
| SublAi, S.A. de C.V. | |
| Suempresa.com | Mexico City |
| Supercep, S.A. de C.V. | Mexico City |
| Supercomputo On Line, S.A. de C.V. | Oaxaca |
| SuperTasas | Nuevo León |
| Sustam, S.A de C.V. | Jalisco |
| Sustam, S.A. de C.V. | |
| Swap | |
| Swichi Eléctrica de México | Mexico City |
| Sys&Web | Mexico City |
| Sys.Net.Mx, S. de R.L. de C.V. | Mexico City |
| Syscap | |
| Sysne | |
| SysOp | |
| T&M Consulting | |
| T-Systems México | Mexico City |
| Tachuela Marketing | |
| Tagwizz | Mexico City |
| Talent Land | |
| Talent Network | Jalisco |
| Talently | |
| Talisis Holding, S.A.P.I. de C.V. | |
| Tapuko Marketing | |
| Tarkus | |
| Tasisoft | Mexico City |
| TBANC | Jalisco |
| TBC Universidad | |
| TEC Electronica S.A. de C.V. | Mexico City |
| Tec Gurus | Mexico City |
| Tec Monclova Tec Monclova . Tecnológico Nacional de México Campus Monclova | |
| Tech Minds | |
| TechBA | Mexico City |