| Welldone Software | Tel Aviv District |
| WellyBox | Tel Aviv District |
| WeSell | Tel Aviv District |
| Whatslly | Tel Aviv District |
| Whist | Tel Aviv District |
| White-Hat | Tel Aviv District |
| Wicked Brands | Tel Aviv District |
| WildWeb | Tel Aviv District |
| Wiliot | Haifa District |
| Windward Ltd | Tel Aviv District |
| Wing Security Ltd | Tel Aviv District |
| WINN | Tel Aviv District |
| Wise Communications Solutions | Tel Aviv District |
| Wise Solutions LG Ltd | |
| WiseInfo | Northern District |
| Wisor.ai | Tel Aviv District |
| Wisy | |
| Wix.com, Inc. | Tel Aviv District |
| Wiz | Tel Aviv District |
| Wizdome Ltd | |
| WizSoft, Inc. | Tel Aviv District |
| Wizzo | Jerusalem District |
| Wobi | Tel Aviv District |
| WonderLogics Ltd | Northern District |
| WonderNet Ltd | Central District |
| wwO | Tel Aviv District |
| WYZE | Central District |
| XACT Robotics Ltd | Haifa District |
| Xenia Venture Capital | Southern District |
| xFind | Tel Aviv District |
| Xhost | Tel Aviv District |
| XM Cyber | Tel Aviv District |
| XPlace Ltd | Tel Aviv District |
| Xplenty | Tel Aviv District |
| Xrom Group | |
| Xsight Labs Ltd | Southern District |
| Xsight Systems Ltd | Central District |
| xSite Communications | Tel Aviv District |
| XTL Biopharmaceuticals Ltd | |
| Xtra Mile | Central District |
| Yaaf | Tel Aviv District |
| Yael Group | Tel Aviv District |
| Yamar Electronics Ltd | Tel Aviv District |
| YASKAWA Europe Technology Ltd | |
| Yeda Research & Development Co., Ltd | |
| Yezreel Valley College | |
| YG Design | Tel Aviv District |
| Yissum Technology Transfer Company of the Hebrew University | Jerusalem District |
| YIT | Central District |
| Yoom.com, Inc. | Tel Aviv District |