| IriusRisk | Huesca |
| Irontec | Biscay |
| IrsiCaixa | |
| isardSAT | Barcelona |
| ISDI Accelerator | Madrid |
| ISDI Digital Talent | Madrid |
| iSeazy | Madrid |
| iSencia, S.L. | Barcelona |
| iSeo + Marketing | |
| Isigma Asesoría Tecnológica, S.L. | Barcelona |
| IslaWEB, S.L. | Balearic Islands |
| IspActiv | Barcelona |
| Istituto Europeo di Design S.p.A. | |
| IT Smart Finance Investment, S.L. | Barcelona |
| it&people, S.L. | Madrid |
| IT-Design | Málaga |
| IT2B | |
| Itaktion, S.L. | Gipuzkoa |
| ITCL Centro Tecnológico | |
| ITCOM Telecomunicaciones, S.L.U. | Madrid |
| ITcSystem | Madrid |
| Iteisa | Cantabria |
| Item Multimedia, S.L. | Madrid |
| Itemvirtual, S.L.U. | Barcelona |
| Itera, S.L. | |
| Iteralix Enterprise, S.L. | Barcelona |
| ITGLOBAL | Barcelona |
| ITH Network, S.L. | Toledo |
| IThinkUPC | Barcelona |
| Itnig | Barcelona |
| ITSOFT | Sevilla |
| Ivanovich Games, S.L. | Barcelona |
| iVascular, S.L.U. | Barcelona |
| Ivigo Business School | Pontevedra |
| IVO Fundación Instituto Valenciano de Oncología | |
| iwan21 | Sevilla |
| IzarMicro | Biscay |
| Izertis, S.A. | Asturias |
| JAD Solucions Informàtiques, S.L. | Barcelona |
| Jaguar Path Ventures | Madrid |
| Jamgo | |
| Jarvis Sistemas Inteligentes, S.L. | Madrid |
| Java4Less.com | Valencia |
| Javier Gosende | Alicante |
| JC Informática | Madrid |
| JCC Suministros y Sistemas Informáticos, S.L. | Madrid |
| JDM Correduria de Seguros, S.L. | Barcelona |
| Jensen Technologies, S.L. | Granada |
| Jensen-Localization, S.L. | Málaga |
| Jet Computer | Madrid |