| elsa.care | Madrid |
| Elson Electrónica, S.A. | Biscay |
| Eltec IT Services, S.L. | Madrid |
| EMAGISTER Servicios de formación, S.L. | |
| eMailing Force | Madrid |
| eMascaró | Barcelona |
| Embacy | |
| Embat | Madrid |
| Embedded instruments and Systems, S.L. | Alicante |
| Embention | Alicante |
| Emenia | Madrid |
| Emergento | Las Palmas |
| Emergya | Sevilla |
| Emérita Legal | |
| Emesa, S.L. | La Rioja |
| Èmfasi | Barcelona |
| Empatica Internet, S.L. | Tarragona |
| Empresawww.com | Valencia |
| Emprosoft, S.A. | Madrid |
| EMXYS | |
| Enagás, S.A. | |
| Enantia | Barcelona |
| Encamina | Valencia |
| Encode Web, S.L. | Madrid |
| Encomenda | |
| Encora | |
| EndoLipiD Therapeutics | |
| Eneo Tecnología, S.L. | Biscay |
| Enetic Proyectos, S.L. | |
| Enfoque Soluciones TI, S.L. | Valladolid |
| Enfusión, Consultoría y Comunicación, S.L. | Barcelona |
| Engel Axil | Barcelona |
| Enghouse Interactive, S.L.U. | Barcelona |
| Engine Software | Barcelona |
| EnginyWorks Technologies, S.L.U. | Barcelona |
| EngrApp | Madrid |
| Enium | Alicante |
| Enreda S.C.A. | Sevilla |
| Ensalza | |
| Entelgy Consulting, S.A. | Madrid |
| Enterprise Europe Network-GalacteaPlus | |
| Entersystems | Barcelona |
| Entorno Digital, S.A. | Barcelona |
| Entornweb | Barcelona |
| Entra en la Red | Madrid |
| Entumovil.net | Barcelona |
| EÑUTT comunicación | Biscay |
| ENXENIO, S.L. | A Coruña |
| Eol Systems, S.L. | Barcelona |
| ePages Southern Europe, S.L. | Barcelona |