| Uddannelsescenter Holstebro | |
| Uizard | |
| Umbraco A/S | Region of Southern Denmark |
| Unconventional Ventures | |
| Understory A/S | |
| Undo Forsikringsagentur A/S | Capital Region of Denmark |
| UNEEG medical | |
| Unica Host ApS | Region Zealand |
| Unifeeder A/S | |
| Uniify ApS | |
| Unik System Design A/S | Region of Southern Denmark |
| UNION therapeutics A/S | |
| Uniqkey A/S | |
| Unique Human Capital A/S | Capital Region of Denmark |
| Unisense A/S | |
| Unit IT | |
| UnitSpeed Host | Capital Region of Denmark |
| Universal Robots A/S | Region of Southern Denmark |
| UNIwise ApS | |
| Unsilo | Central Denmark Region |
| Upodi ApS | |
| Upteko ApS | |
| Urbistic | |
| UserTribe | Capital Region of Denmark |
| UV Medico A/S | |
| UVdata A/S | |
| Valuer.ai | |
| Var2 Pharmaceuticals ApS | |
| vConnect | |
| vConnect | |
| Vejle RC Elektronik ApS | Region of Southern Denmark |
| VenomAid | |
| VentriJec | |
| Venture Cup Denmark | |
| VentureScout Ltd | |
| Veo Technologies ApS | |
| Vesper Bio | |
| Vestas Wind Systems A/S | |
| VestNet ApS | |
| VIA University College | Central Denmark Region |
| ViaBill | |
| Vibrant ApS | |
| Viden Djurs | |
| Viewsource.dk | Capital Region of Denmark |
| Viggo HQ ApS | |
| ViiaBill | |
| Viio Technologies ApS | |
| Vindstød A/S | |
| Vipergen ApS | |
| Virsabi ApS | Capital Region of Denmark |