| sima.dk A/S | Region of Southern Denmark |
| SimaTech A/S | |
| SimCorp | Capital Region of Denmark |
| Simple Solution P/S | |
| SimpleSite | Capital Region of Denmark |
| Simply.com | Capital Region of Denmark |
| Simsoft | |
| SITECH Danmark A/S | |
| Siteimprove A/S | |
| Skanderby A/S | Central Denmark Region |
| Skåne Sjælland Linux Users Group | Region Zealand |
| Skive College | Central Denmark Region |
| Skjoldby & Co ApS | Region Zealand |
| Skycore Semiconductors | |
| Skycore Semiconductors | |
| Skysolution ApS | |
| Sl-software | Capital Region of Denmark |
| Sleeknote ApS | |
| Slize Commerce ApS | |
| SmartResponse | |
| SmartShare Systems A/S | Capital Region of Denmark |
| SmartTID | Region of Southern Denmark |
| SMT Data A/S | |
| Snipr Biome | |
| Soft Design | Capital Region of Denmark |
| SoftBASE A/S | |
| SoftControl | |
| Softpay ApS | |
| SoftwareONE Denmark Aps | |
| Softwork | Region Zealand |
| Solid Therapeutics | |
| Solitwork | |
| Solution Group ApS | |
| Solution IT ApS | |
| SolutionWeb | Capital Region of Denmark |
| Somatic ApS | |
| SoMeWeb | |
| Sopra Steria AS | Capital Region of Denmark |
| Sorthvid | |
| Soundblocks | |
| Space Composite Structures DENMARK ApS | |
| Space Inventor ApS | |
| SPAMfighter | Capital Region of Denmark |
| Sparekassen Danmark | |
| Sparekassen Kronjylland | |
| Sparekassen Sjælland-Fyn A/S | |
| SparePart.dk | Region of Southern Denmark |
| Sparrow Quantum ApS | |
| Spectra Systems | Region of Southern Denmark |
| Spectro Inlets ApS | |