| Lindgren Automation | Ontario |
| Line-Tap Computer Service | Ontario |
| LineaBio | |
| Linearis | |
| Link Computer | Ontario |
| Link Investment Management, Inc. | |
| Link Telecom | Ontario |
| Link Three Media | Ontario |
| Link Web Development Ltd | Ontario |
| Linkage Electronics | Ontario |
| Linke10, Inc. | Ontario |
| Linkeo | Quebec |
| Linko Solutions | Quebec |
| LinkPoint IT Solutions Ltd | |
| Links Without Borders | British Columbia |
| LinkTiger, Inc. | British Columbia |
| Linky Product | |
| Linovati | |
| Linowski Interactive Design | Ontario |
| LINUEN | |
| LinuQ | Quebec |
| Linux Professional Institute | Ontario |
| Linux-Ottawa | Ontario |
| Linux/Open Source Users of Regina, Saskatchewan, Inc. | Saskatchewan |
| Linuxcare.ca | British Columbia |
| LinuxMagic | British Columbia |
| Linxpot, Inc. | |
| LinxSmart, Inc. | Ontario |
| Linxtal | |
| Lion Eater | |
| Lion Électrique | Quebec |
| Lionhardt Technologies | Ontario |
| Liontech Solutions, Inc. | British Columbia |
| Lios Technologies, Inc. | |
| Liquid Analytics | |
| Liquid Avatar Technologies, Inc. | |
| Liquid Capital Corporation | Ontario |
| Liquid Labs, Inc. | Ontario |
| Liquid Media | Nova Scotia |
| Liquid Sound | British Columbia |
| Liquid Trends Media | Alberta |
| LiquidCMS | Ontario |
| Liquidity Services, Inc. | Ontario |
| Liquify | |
| LIR Life Sciences | |
| LISA | Ontario |
| Lisa Gillis | |
| Listed Technologies, Inc. | |
| ListMate | Nova Scotia |
| Litcom | Ontario |