| Liberty & Associates | Quebec |
| Liberty Security | Alberta |
| Liberty Street Software | Ontario |
| Liberty Village IT | Ontario |
| Libra Information Technologies | Ontario |
| Life Sciences British Columbia | |
| Life Sciences Nova Scotia | |
| Life Sciences Toronto | |
| Life's a Glitch | Ontario |
| LifeBooster | |
| LifeGuide | Ontario |
| Lifeline Design | Ontario |
| Lifeline Total I.T. Solutions, Inc. | Ontario |
| LifeScan Canada Ltd | British Columbia |
| LifeSpeak, Inc. | Ontario |
| Lifestyle Communications | |
| LifeStyleMedia | |
| LifeTrack Safety Systems Ltd | |
| Lift Interactive | Alberta |
| LiftAccelerator | |
| Lifter.ca | |
| Liftoff Capital | |
| Light Speed Web | Ontario |
| Light Studio | Quebec |
| Light Switch Creative | |
| Lightbody Marketing | Ontario |
| Lightbox Technologies, Inc. | Ontario |
| LightCode, Inc. | Ontario |
| Lighthouse Labs | British Columbia |
| LightMachinery, Inc. | Ontario |
| Lightning Circuits, Inc. | Ontario |
| Lightning Computer Consultant Service | British Columbia |
| Lightning IT Services | Alberta |
| Lightning Rod Games, Inc. | Ontario |
| Lights On Software | Ontario |
| Lightspeed Communications | British Columbia |
| Lightspeed POS, Inc. | Quebec |
| Lightwave Computing Ltd | British Columbia |
| Liifer | |
| Like 10, Inc. | Ontario |
| Likuid.com | Quebec |
| Lim Geomatics | Ontario |
| Limbus AI, Inc. | |
| Limelight Marketing | British Columbia |
| Limelight Platforms, Inc. | |
| Limelight Software | |
| LimeSpot | |
| Liminal BioSciences, Inc. | |
| Limosa, Inc. | |
| Lincoln-Parry SoftEscrow, Inc. | Ontario |