| Défi Innovation Estrie | Quebec |
| Define Capital | |
| Definite Image Productions, Inc. | Quebec |
| Defuse Security | |
| Degama Systems, Inc. | Ontario |
| Degroot Design | Ontario |
| Dejero Labs, Inc. | |
| DEK Technologies | Alberta |
| DELAN | |
| Delastek, Inc. | Quebec |
| Delco Automation, Inc. | Saskatchewan |
| Delecta Technologies, Inc. | |
| Délégation Informatique | |
| Delego Software, LLC | Ontario |
| Delex Media Ltd | |
| Delisoft | Quebec |
| Delivery Net Canada, Inc. | |
| DeliverySuite | |
| Dell Refurbished | Ontario |
| Dell Smart Home Solutions | Ontario |
| Delo Logic | Ontario |
| Delphia Technologies, Inc. | |
| Delron Laser, Inc | Alberta |
| Delsoft | Quebec |
| Delta 360, Inc. | Ontario |
| Delta Growth | |
| Delta Photonics | Ontario |
| Delta-Q Technologies | |
| Deltageeks | British Columbia |
| deltAlyz | |
| Deltatee Enterprises Ltd | Alberta |
| Deluan Quintão | |
| Delusional Marketing Company | |
| Deluxe Site Builder | British Columbia |
| DelveIn Analytics, Inc. | |
| DelveTek Consulting | Ontario |
| Delvinia Group of Companies | Ontario |
| Demand Strategies Group, Inc. | Ontario |
| DemandHub, Inc. | |
| DeMarque | Quebec |
| Demca Technologies | Quebec |
| DeMondes.com | Quebec |
| Demotic Design, Inc. | Ontario |
| Dempton Group Conseil | Quebec |
| Dendory Capital | Quebec |
| DendroTEK Biosciences, Inc. | |
| Denman Digital, Inc. | British Columbia |
| Denologix, Inc. | Ontario |
| Denote | Nova Scotia |
| DeNova | |