| TSI | Gauteng |
| TSIBA Education | Western Cape |
| TuksNovation | Gauteng |
| TurboCAD | Gauteng |
| Turfspot (Pty) Ltd | Western Cape |
| Turnup Pin (Pty) Ltd | |
| TuxSoft | Western Cape |
| TWHosting | Western Cape |
| Two Fishes Design | |
| TypeDev | |
| Tyrmida | Gauteng |
| UAASA | Gauteng |
| uAfrica.com | Gauteng |
| Ubuntu Online | Gauteng |
| UCT Graduate School of Business | Western Cape |
| UCT GSB Solution Space | Western Cape |
| UCT Lung Institute | |
| Ukheshe Technologies | Gauteng |
| Ukuvuma Solutions | Gauteng |
| Ultimate Linux Solutions | Gauteng |
| Ultitec Electronics | KwaZulu-Natal |
| Ultra Quick Host | Mpumalanga |
| Umgungunglovu TVET College | |
| Unibase Solutions | Gauteng |
| Uniclox | Gauteng |
| Unicom Computer Services | Gauteng |
| Unified Data | KwaZulu-Natal |
| Unilynx | Western Cape |
| Unipos Point of Sale | Free State |
| UniSource Software Services | Gauteng |
| Uniterm Direct | Gauteng |
| Universal Information Technologies | Gauteng |
| Universal Knowledge Software | Gauteng |
| Universe Direct | Western Cape |
| Universiteit Stellenbosch - Department of Mathematics | Western Cape |
| Universiteit Stellenbosch - Electrical and Electronic Engineering | Western Cape |
| University of Cape Town | Western Cape |
| University of Cape Town - Computational Biology | Western Cape |
| University of Cape Town - Department of Computer Science | Western Cape |
| University of Cape Town - Department of Electrical Engineering | Western Cape |
| University of Cape Town - Department of Mathematics and Applied Mathematics | Western Cape |
| University of Fort Hare | |
| University of KwaZulu-Natal | |
| University of KwaZulu-Natal - Quantum Research Group | |
| University of KwaZulu-Natal - School of Computer Science | KwaZulu-Natal |
| University of KwaZulu-Natal - School of Mathematical Sciences | KwaZulu-Natal |
| University of Limpopo | |
| University of Mpumalanga | Mpumalanga |
| University of Pretoria | |
| University of Pretoria - Department of Computer Science | Gauteng |