| Université Saint-Louis - Bruxelles | Brussels-Capital |
| Universiteit Antwerpen | |
| Universiteit Antwerpen - Informatica | Antwerp |
| Universiteit Gent | East Flanders |
| Universiteit Gent - Computational Web Intelligence Lab | East Flanders |
| Universiteit Gent - Bioinformatics and Systems Biology | East Flanders |
| Universiteit Gent - Vakgroep Wiskunde | East Flanders |
| Universiteit Hasselt | Limburg |
| University de Liège - Montefiore Institute | Liège |
| UniWeb B.V.B.A. | Flemish Brabant |
| UPARTNER | |
| Upper Side | Brussels-Capital |
| Upthrust | |
| Urbantz S.A. | |
| Use IT Group | West Flanders |
| Use Webdesign | West Flanders |
| uWare Robotics | |
| UXpro | |
| V-Bio Ventures | |
| V.L.V. S.A. | Liège |
| ValBiom A.S.B.L. | Namur |
| Van Hoecke Automation | East Flanders |
| VanDongen.NetTechnology B.V.B.A. | Flemish Brabant |
| Vanksen | |
| Vanroey | Antwerp |
| VaultSpeed | |
| Vcomm Ricoh | Flemish Brabant |
| VCS | Flemish Brabant |
| Veezo | Walloon Brabant |
| VEN Brussels | Brussels-Capital |
| Venly | |
| Venture Spirit | |
| Veolia | |
| Veoware | |
| Verassnet S.A. | Flemish Brabant |
| Verco N.V. | Walloon Brabant |
| Verhaert Digital Innovation | |
| Verheecke Internet Services B.V. | Antwerp |
| Vericomp | West Flanders |
| VERIXI | Walloon Brabant |
| Verlinvest | |
| VersaSense | |
| Vertige A.S.B.L. | Brussels-Capital |
| Vertuoza | |
| Vésale Bioscience | |
| Vesalius College Brussels | Brussels-Capital |
| VIB | |
| Victoury B.V. | |
| VIL | |
| Vipcomputers B.V.B.A. | Limburg |