| Autentica | Zug |
| Auterion AG | Zurich |
| Autexis Holding AG | Aargau |
| Authena | |
| Authress | |
| AutoForm Engineering GmbH | Zurich |
| Automation and Control Systems AG | St. Gallen |
| AV Alliance | |
| Ava AG | Zurich |
| Avado | Zug |
| Avallain AG | |
| Avaloq Evolution AG | Zurich |
| Avantec AG | Zurich |
| AVAtronics S.A. | |
| Aveniq AG | Aargau |
| AVIK Systems | |
| Avintis S.A. | Fribourg |
| Avodata AG | Zurich |
| Avrion Therapeutics S.A. | |
| Avrios International AG | |
| AWARDIC GmbH | St. Gallen |
| AWK Group S.A. | Zurich |
| Axalbion S.A. | |
| Axeba AG | Zurich |
| Axeon | Solothurn |
| Axianet.ch S.à.r.l. | Valais |
| Aximetria GmbH | |
| Axino Solutions AG | |
| Axisbits | |
| Axpo Holding AG | |
| Axxiome | |
| Azad Pharma AG | |
| Azone | Zurich |
| b-rayZ AG | |
| b-sharpe | |
| B-works | |
| b/ackcode_ S.A. | |
| Baccinex S.A. | |
| Bachem AG | |
| Bachmann Engineering AG | Aargau |
| Bachmann Support GmbH | Zurich |
| BackBone Ventures AG | |
| Baechler Information S.A. | Fribourg |
| Bainox AG | |
| Baliopharm AG | |
| Banana.ch S.A. | Ticino |
| BantliSolutions | Zurich |
| Barox Kommunikation AG | Aargau |
| Base58 | |
| BaseLaunch | |