| Duomedia | Bratislava |
| Dynatech, s.r.o. | Bratislava |
| Dytron | |
| E-GO, s.r.o. | Trnava |
| e-Sense Slovakia, s.r.o. | |
| E-zone Technologies, s.r.o. | Košice |
| eCall Slovakia, s.r.o. | Bratislava |
| ECOCAPSULE | |
| Edas, s.r.o. | |
| eDoma | |
| EEA | Bratislava |
| eFabrica, a.s. | |
| EFN plus, s.r.o. | Žilina |
| Ekonomická univerzita v Bratislave | |
| Ekovital, s.r.o. | |
| Ekusoft.sk, s.r.o. | Trenčín |
| ELBIA, s.r.o. | Banská Bystrica |
| ELCID Group, s.r.o. | |
| Electronik Servis, spol. s r.o. | Bratislava |
| Elektrotechnický ústav SAV, v.v.i. | |
| ELET, s.r.o. | Bratislava |
| Elevon.io j.s.a. | |
| Elfa, s.r.o. | Košice |
| Elis, s.r.o. | |
| Elite Solutions | Bratislava |
| ElitSat, s.r.o. | Bratislava |
| Elnec, s.r.o. | Prešov |
| ELPRO, s.r.o. | Košice |
| Elumi Studio | |
| ELV.ai | |
| Empire Systems, s.r.o. | |
| EMtest, a.s. | |
| EMware a.s. | Žilina |
| Ene-Tep, a.s. | |
| Energia - Jarás, s.r.o. | |
| Energo-Aqua, a.s. | |
| Ensentia, s.r.o. | |
| Entry Net | Bratislava |
| Epix | Trnava |
| ErasData-Pro | |
| ErlaServers, s.r.o. | |
| eROBOT, j.s.a. | |
| ESET, spol. s r.o. | Bratislava |
| ESMO Žilina, a.s. | Žilina |
| eSolutions, s.r.o. | Košice |
| Espio, s.r.o. | |
| ESTEN, s.r.o. | |
| eStranky.sk | Bratislava |
| Etarget | |
| Etarget SE | Bratislava |