| Slovenská technická univerzita v Bratislave | Bratislava |
| Slovenská technická univerzita v Bratislave - Fakulta elektrotechniky a informatiky | Bratislava |
| Slovenská zdravotnícka univerzita | Bratislava |
| Slovnaft | |
| Smart Choice, s.r.o. | |
| Smart Media Star, s.r.o. | |
| smart-MAIC LAB, LLC | |
| SmartBase, s.r.o. | |
| Smartweb, s.r.o. | Nitra |
| Softec | |
| SOFTIP a.s. | Bratislava |
| Softpae | Bratislava |
| SoftPoint, s.r.o. | Bratislava |
| Software Partner, spol. s r.o. | Bratislava |
| Softworx | Žilina |
| Soitron, s.r.o. | Bratislava |
| SoliT, s.r.o. | Bratislava |
| Sorudan Solutions, s.r.o. | Bratislava |
| SOS electronic, s.r.o. | Košice |
| Source-Code | |
| Speekle, s.r.o. | |
| Spinbotics | |
| SQP International, s.r.o. | Banská Bystrica |
| Staffino s.r.o. | Bratislava |
| Stanislav Klubert | Košice |
| Starbug, s.r.o. | Žilina |
| Startitup.sk | |
| Startup centrum | |
| STEP software, s.r.o. | Košice |
| Sting-Energo, s.r.o. | |
| Stix, s.r.o. | Žilina |
| Storm, s.r.o. | |
| Stormware, s.r.o. | Bratislava |
| Studio Style | Bratislava |
| Styleweb | Bratislava |
| SunSoft | Žilina |
| superfaktura.sk, s.r.o. | Bratislava |
| Supermobilioty.SK | Trenčín |
| SuperScale | |
| SVOP, spol. s r.o. | Bratislava |
| SWAN, a.s. | Bratislava |
| Sygic | Bratislava |
| Synapsys, s.r.o. | Bratislava |
| Synetics | Trenčín |
| Syntegra Systems | Bratislava |
| SysCom | Bratislava |
| SYSKOS, s.r.o. | |
| Sysoon, s.r.o. | |
| Systematic, s.r.o. | |
| Systemios Ltd | |