| CzechGamer.com | Prague |
| Czechia24, s.r.o. | Prague |
| CzechInvest | |
| Czechitas z.ú. | |
| Czechproduct.cz, s.r.o. | Olomouc |
| CzechStartups.org | Prague |
| CZSpeed.NET, s.r.o. | Prague |
| D-Soft, s.r.o. | Prague |
| Daktela | |
| Dáme jídlo | |
| Daniel Fiser | Prague |
| DAQUAS, spol. s r.o. | |
| DASTON European Intelligence & Security | Prague |
| Data Collect, s.r.o. | |
| Data Force | |
| Data From Sky | |
| Data Intertech, s.r.o. | |
| Data Labs, s.r.o. | Prague |
| Data Servis - informace, s.r.o. | |
| Data-Inter, spol. s r.o. | |
| DATA-Software, spol. s r.o. | |
| DATA4i, s.r.o. | Hradec Králové |
| Data4You | Prague |
| DataApex | Prague |
| Databox, s.r.o. | |
| DataCentrum systems & consulting, a.s. | |
| Datacon MSI, s.r.o. | |
| Datacruit, s.r.o. | |
| DataKal | |
| DataLite, spol. s r.o. | Prague |
| Datamix Solutions, s.r.o. | Olomouc |
| Datamole, s.r.o. | |
| dataPartner, s.r.o. | |
| Datapro Solutions | Prague |
| DATARECOVERY, s.r.o. | Prague |
| Datas Plus, s.r.o. | |
| Datascan, s.r.o. | |
| DataScript, s.r.o. | |
| DataSession, s.r.o. | |
| Datasoft, spol. s r.o. | |
| Datasys, s.r.o. | Prague |
| DataVision | |
| DATAWEPS services, s.r.o. | |
| Dateio | |
| Datensen | |
| David Nekovář | |
| David Šauer - DS-SOFT | Prague |
| DCfreenet | Prague |
| DeCe COMPUTERS, s.r.o. | Ústí nad Labem |
| Delnet, s.r.o. | |