| BePositive, s.r.o. | |
| Berghof Systems, s.r.o. | |
| BEST FOR NET, s.r.o. | Prague |
| Bestsite, s.r.o. | |
| BeWebly | |
| BIC Brno spol. s r.o. | |
| Billanc Partners ČR, s.r.o. | |
| BinarBase, s.r.o. | |
| BINARGON, s.r.o. | Moravian-Silesian |
| Bioinova, a.s. | |
| Bioinova, a.s. | |
| BiQ Bluesoft, a.s. | |
| BiSigma Česko | |
| Bit CZ Training, s.r.o. | |
| Bitart | South Moravian |
| BitKinex | Liberec |
| Bitperia | |
| BitServis | |
| Bitt technology ČS, spol. s.r.o. | |
| Biz Data, a.s. | |
| BizzTreat | |
| Black Point DEV, s.r.o. | |
| Blackfish & Co., s.r.o. | |
| Bleha Jiří | Prague |
| Blindspot Solutions | |
| Blockchain Hub Prague | Prague |
| Blocshop, s.r.o. | |
| Blocshop, s.r.o. | |
| Blogic | |
| Blueberry | Prague |
| Blueboard.cz, s.r.o. | Prague |
| BlueGhost | Prague |
| BM Software | South Moravian |
| BNP Paribas Czech Republic | |
| Bohemian AI Technologies, s.r.o. | Prague |
| Bolt Start Up Development, a.s. | Prague |
| BONDA, s.r.o. | Prague |
| Booking Analytics | |
| Boomerang Communication, s.r.o. | |
| Boost.space | |
| BOOTIQ | |
| Born Digital | |
| Born Digital, s.r.o. | |
| BossCan ComPrint, s.r.o. | South Moravian |
| Boxart, s.r.o. | |
| BPdata, spol. s r.o. | Prague |
| Brackmedia | |
| Braiins | |
| BRE.cz | Moravian-Silesian |
| Brightify | South Moravian |