| Fitify Workouts, s.r.o. | |
| Flatio, s.r.o. | |
| Flex Databases, s.r.o. | |
| Flowpay, s.r.o. | |
| FlowUp | |
| FlutLab, s.r.o. | |
| Forecom | Prague |
| Forpsi Internet CZ, a.s. | Prague |
| Fortion Networks, s.r.o. | Plzeň |
| FOTON, s.r.o. | |
| Fragaria, s.r.o. | Prague |
| Fram Software, s.r.o. | Prague |
| FreeHosting | Prague |
| Frentech Aerospace, s.r.o. | |
| FULLCOM systems, s.r.o. | |
| FutLab.cc | |
| Future Port Prague | Prague |
| Future Rockstars | |
| Futured | South Moravian |
| Fyzikální ústav AV ČR, v. v. i. | |
| G.L.Electronic, s.r.o. | |
| Galance.NET | Central Bohemian |
| GC System | South Moravian |
| GDI, s.r.o. | South Moravian |
| GEDIP | Zlín |
| GeekCoders | |
| Geetoo Holding, a.s. | Prague |
| Geewa | |
| Geewa, a.s. | Prague |
| GENEEA Analytics, s.r.o. | |
| GeneProof, a.s. | |
| General AI Challenge | Prague |
| General Bytes, s.r.o. | |
| Generi Biotech, s.r.o. | |
| Genesis Capital, s.r.o. | Prague |
| Gentleface, s.r.o. | Prague |
| GEPRO, spol. s r.o. | Prague |
| GES-ELECTRONICS, a.s. | Plzeň |
| GESTO Computers, spol. s r.o. | Prague |
| Giant interactive, s.r.o. | Prague |
| Gigaweb.cz | Hradec Králové |
| GINA Software, s.r.o. | |
| Gisat, s.r.o. | |
| GISoft | Moravian-Silesian |
| GiTy, a.s. | South Moravian |
| GLOB Software, s.r.o. | |
| GLOBDATA, a.s. | |
| Globula | |
| Glueo, s.r.o. | Prague |
| Golang.cz, s.r.o. | |