| Roud Studio | Masovian |
| Routine Automation | |
| Roxart Agency | |
| ROXX Media | |
| Royal Company Sp. z o.o. | |
| RSI | Silesian |
| RST LIFT-OFF Startup Accelerator | |
| RST Software Masters | Lower Silesian |
| RST Sp. z o.o. | |
| RST Ventures For Earth Sp. z o.o. | |
| Ruby Logic | Silesian |
| Rubyroid Labs | |
| Rumble Fish | Lesser Poland |
| Run By IT Sp. z o.o. | |
| Rybnet | Silesian |
| RYVU Therapeutics | |
| S 90 | Silesian |
| S&P Partners | |
| S-Labs | |
| S-NET Sp. z o.o. | Lesser Poland |
| S.O.S. P.M. Wesołowscy | Kuyavian-Pomeranian |
| S4E S.A. | |
| SaaS Native Sp. z o.o. | |
| SABUR Sp. z.o.o. | Masovian |
| Sacer S.C. | Pomeranian |
| Safebox24 | |
| SaferInternet | Masovian |
| SafetyHeads | |
| Saffron Sp. z o.o. | |
| SafiStudio | |
| Sages Sp. z o.o. | Masovian |
| Sagiton | |
| Sailing Byte Sp. z o.o. | |
| Saleor | |
| SALESmanago | |
| SALESmanago | |
| Saly | |
| Samito S.A. | |
| Samsung Electronics Polska Sp. z o.o. | Masovian |
| Sanddev | |
| Sanmar Sp. z o.o. | |
| SATIM | |
| Satisfy | |
| SatRev | |
| SaturdaysHeroes Sp. z o.o. | |
| Satus | |
| Satus Venture Sp. z o.o. | Lesser Poland |
| Saule Technologies | |
| Saving Cloud | |
| Sawicom | Greater Poland |