| Autonomous Knight B.V. | |
| Autoriteit Consument & Markt | |
| Autoriteit Financiële Markten | |
| Autoriteit Persoonsgegevens | South Holland |
| Autoscriber | North Brabant |
| Autosoft B.V. | Overijssel |
| Avahe Telecommunicatie B.V. | South Holland |
| Avalon IT | |
| Avania | Utrecht |
| Avans Hogeschool | North Brabant |
| Avant Webdiensten | South Holland |
| Avantium | |
| Avedon Capital Partners B.V. | North Holland |
| Avetica B.V. | South Holland |
| Avicenna Health Hack | |
| Avidicure | |
| Avineon Tensing | |
| Avisi B.V. | Gelderland |
| Avit Group | Utrecht |
| Avitus | North Holland |
| Aviva Solutions | South Holland |
| Avivia B.V. | |
| Avy | |
| Awaretrain | Gelderland |
| Axelera AI | North Brabant |
| Axign | |
| Axiom Information Technology | Gelderland |
| Axion | Groningen |
| Axivate Capital | North Holland |
| Axivorm internet & publicity | Friesland |
| Axoft IT & Telecom | South Holland |
| Axon Digital Design B.V. | North Brabant |
| AxonIQ | |
| Axtron B.V. | Utrecht |
| Axxem B.V. | |
| aYniK Software Solutions B.V. | Overijssel |
| Azafaros | North Holland |
| Azavista | |
| Azerion | North Holland |
| Azor | North Holland |
| Aztlán | North Holland |
| B-Lex IT B.V. | Overijssel |
| B-ware Business Software | Overijssel |
| B.A.C. | South Holland |
| B3 Industrie B.V. | North Brabant |
| Baas & Basse | |
| Babylonia Vertalingen | Friesland |
| Backshop Healthy Computing B.V. | South Holland |
| Backup in Business | North Brabant |
| Bakker & Spees | North Holland |