| Iristrace B.V. | |
| IrriWatch | Gelderland |
| Isah | North Brabant |
| ISD | |
| Iselinge Hogeschool | |
| Isential | Overijssel |
| ISISPACE Group | |
| Islamic University of Applied Sciences Rotterdam | |
| ISM eCompany | South Holland |
| iSnapp | |
| ISOCARP | |
| ISPWeb B.V. | |
| ISSYS ICT B.V. | North Holland |
| IT Aces Industrial Automation | South Holland |
| IT Consultancy Group B.V. | Utrecht |
| IT's Logic | Gelderland |
| IT-Button.com B.V. | |
| IT-Contracts.nl | Utrecht |
| IT-Recycling | North Brabant |
| it-solid | Flevoland |
| IT4Success | South Holland |
| ITB2 Datacenters | Gelderland |
| ITcetera | Gelderland |
| ITIS | South Holland |
| ItKin Design | Friesland |
| ITpreneurs | South Holland |
| ITR Automatisering | |
| ITSD | North Holland |
| Itude Mobile B.V. | |
| ITurnITy Information Group B.V. | North Holland |
| ITV Hogeschool voor Tolken & Vertalen | |
| itWebservice | North Holland |
| It’s Us B.V. | North Holland |
| IVA Business School | |
| Ivium Technologies B.V. | North Brabant |
| iWAD Support & Service op 1 | North Brabant |
| iWebDesign | North Brabant |
| iWink | Groningen |
| iX Studios | |
| Ixcon(r) B.V. | North Brabant |
| Ixon B.V. | |
| iXware | South Holland |
| iZito | |
| J&BCS | Utrecht |
| Jacobs Development B.V. | |
| Jalt | North Holland |
| JamCrackers | Flevoland |
| Jansen Internetservice | |
| Janssens Kassasysteme | Gelderland |
| JanusID B.V. | |