| Adyen | North Holland |
| AE-ICT B.V. | Gelderland |
| AEG Power Solutions B.V. | North Holland |
| Aegon | |
| AEMICS B.V. | Overijssel |
| Aeon Service ICT | South Holland |
| AerCorp Holdings B.V. | |
| AerData B.V. | North Holland |
| Aeres Hogeschool | Flevoland |
| Aeres Training Centre | |
| Aerial Precision B.V. | |
| Aescap Ventures | North Holland |
| Aexus | |
| AFAS Software | Utrecht |
| Affidea | |
| AffinIT | Groningen |
| African Clean Energy B.V. | |
| AfterImage | South Holland |
| AG5 | North Holland |
| AGCS.works | |
| Agcurate B.V. | |
| Agenda | |
| AGILeBiotics B.V. | |
| AGN | Gelderland |
| AGO Jobs & HR | |
| AgriDirect B.V. | Limburg |
| Agrippa | Limburg |
| Agro Green Systems | |
| Agro IT B.V. | Gelderland |
| AgXeed B.V. | |
| AI in Motion | |
| AI Lab One | South Holland |
| AI Training Nederland | |
| AICN Automatisering | Utrecht |
| Aiden | |
| Aiden Netherlands B.V. | Utrecht |
| Aidence | North Holland |
| Aideon Webdesign | North Holland |
| Aiir Innovations | |
| Aimedis B.V. | |
| Aimforthemoon | North Holland |
| AimValley B.V. | North Holland |
| Aiosyn | Gelderland |
| Airborne | South Holland |
| Airbus Netherlands B.V. | |
| Airchip B.V. | |
| Airport Telecom | North Holland |
| AIS-Scanology B.V. | North Brabant |
| AISEO | |
| AISLER B.V. | |