| Amblique | New South Wales |
| Amdex | New South Wales |
| AMIRE Digital Marketing Services | New South Wales |
| AMPL Software | New South Wales |
| Amplia Therapeutics Ltd | Victoria |
| Amplified Engineering Pty Ltd | Western Australia |
| Amristar Solutions Pty Ltd | Western Australia |
| Amy Who? | South Australia |
| Analysis-one | Queensland |
| Anata Digital | Western Australia |
| Anatomics Pty Ltd | Victoria |
| Anchor Digital | Queensland |
| Anchor Systems | New South Wales |
| Andelo | New South Wales |
| Anderson Morgan | Tasmania |
| Andor Information Technology | New South Wales |
| Andromeda | Victoria |
| Andzen | Queensland |
| Angry Koala Pty Ltd | New South Wales |
| Anixis | New South Wales |
| Anomaly | South Australia |
| Another World Computer Centre | Victoria |
| ANS Communications Pty Ltd | New South Wales |
| Ansarada | New South Wales |
| Anspired Pty Ltd | Queensland |
| Antenna Direct | Western Australia |
| Anteris Technologies | Queensland |
| Anthony Kolber | Victoria |
| Anthrocell Pty Ltd | New South Wales |
| Anthrocell Pty Ltd | New South Wales |
| Antisense Therapeutics Ltd | Victoria |
| AOS Group | Victoria |
| APCD | New South Wales |
| Apex Digital Pty Ltd | Queensland |
| Apex Pacific | New South Wales |
| Apexhost Pty Ltd | Victoria |
| APLDigital | New South Wales |
| Aplus Technology Pty Ltd | New South Wales |
| APNIC | Queensland |
| App Boxer | New South Wales |
| App Gurus | Queensland |
| App Media | New South Wales |
| Appcon Pty Ltd | New South Wales |
| Appello Pty Ltd | New South Wales |
| Appen | New South Wales |
| Appen Ltd | |
| Appetiser Pty Ltd | Victoria |
| Apple Advertising Pty Ltd | |
| Apple Bondi | New South Wales |
| Apple Castle Towers | New South Wales |