| Lighterra | Queensland |
| Lighthouse Business Innovation Centre | Australian Capital Territory |
| Lightplay | New South Wales |
| Limelight Galaxy | |
| Limepay | New South Wales |
| Limewire Gaming Australia Pty Ltd | |
| Lindentech | |
| LinearG | New South Wales |
| Link Digital | Australian Capital Territory |
| LinkageWiz | South Australia |
| Linktree | Victoria |
| LINQPad Pty Ltd | Western Australia |
| Linux Australia | New South Wales |
| Linux Users of Victoria | Victoria |
| Linux Webhost | New South Wales |
| LinuxNow | |
| Liquid Agency Pty Ltd | Victoria |
| Liquid Digital | |
| Liquid State | |
| Liquid Vision | New South Wales |
| Listium Pty Ltd | |
| Little City Coworking | South Australia |
| Little Dash | |
| Little Green Pharma Ltd | |
| Little Hotelier | |
| Littlepay | Victoria |
| Liveable Insights Pty Ltd | New South Wales |
| LiveHire | Victoria |
| Liven Pty Ltd | |
| Living Room of Satoshi Pty Ltd | |
| Lizzy Internet | Victoria |
| Loam Bio Australia | |
| Loc8 | Victoria |
| Locafy | |
| Local Measure | New South Wales |
| Local SEO Perth | Western Australia |
| Localnode Solutions | New South Wales |
| Logic Squad | South Australia |
| LogicalTech Group | Victoria |
| Logicsofts Australia Pty Ltd | |
| LogoGarden.com Pty Ltd | Queensland |
| Logomotion Web | Queensland |
| LogoPeople Australia | New South Wales |
| Long Distance Technologies | South Australia |
| Longpela Expertise | Western Australia |
| Lookahead | New South Wales |
| Loop Secure | Victoria |
| Loop11 | Victoria |
| Loopli | |
| Lorenzo Bocchi | |