| InterValley Ventures | Victoria |
| Interworld Electronics | Victoria |
| Intesols | |
| Intitio Pty Ltd | New South Wales |
| InTouch Web Services | New South Wales |
| Intra Tec | New South Wales |
| Intrac Australia Pty Ltd | New South Wales |
| Intresto Pty Ltd | New South Wales |
| Intuition SofTech Australia | |
| Intuitive IT | |
| Intuitive IT | |
| Intuji | Western Australia |
| Inventia Life Science | |
| Inventia Life Science Pty Ltd | |
| Inventient | |
| Inveress Web Development | South Australia |
| Invest Victoria | Victoria |
| Investible | |
| Investible Pty Ltd | New South Wales |
| Investment NSW | New South Wales |
| Invex Therapeutics | |
| Invigor Group | New South Wales |
| Invisible Ink Pty Ltd | New South Wales |
| INX Software | |
| Ionata Digital | Tasmania |
| iOnline Pty Ltd | Queensland |
| IONYX Digital | |
| IoT Alliance Australia Ltd | New South Wales |
| iP Edge | New South Wales |
| IP Telco | Queensland |
| IP Trading Pty Ltd | New South Wales |
| IPP Technologies | |
| iPrimus Telecommunications Pty Ltd | Victoria |
| iProgrammer | |
| ipSCAPE | New South Wales |
| IQ PC Services Pty Ltd | Western Australia |
| IR | New South Wales |
| iRealty | Queensland |
| Ireckon Pty Ltd | Queensland |
| IREN | |
| Iress | Victoria |
| Ironbark Software | Queensland |
| IRT Electronics Pty Ltd | New South Wales |
| ISA Technologies | Western Australia |
| Isentia | New South Wales |
| ISH Technologies | |
| ISI Australia | New South Wales |
| Island Pharmaceuticals | |
| iSmart Software | Queensland |
| iSmarts | |