| Enterprise Monkey | Victoria |
| Entire Tech | |
| Entity Data Pty Ltd | Queensland |
| eNTITy1 Pty Ltd | |
| Ento | Victoria |
| Entra Tech | |
| Entrée Recruitment | South Australia |
| Envato Elements Pty Ltd | |
| Envato Pty Ltd | Victoria |
| Envato Tuts+ | Victoria |
| Envious Technology Pty Ltd | Victoria |
| Envisage Apps | |
| Envisage Training Pty Ltd | Queensland |
| Enzyme Labs | |
| EonX | Victoria |
| Epic IT | Western Australia |
| Epichem Pty Ltd | Western Australia |
| Epiminder Ltd | |
| EPM Partners | New South Wales |
| EPS | Victoria |
| Epson Australia Pty Ltd | New South Wales |
| EQG Computers | New South Wales |
| Equator IT | Queensland |
| Equatorial Launch Australia | |
| Equiem Services Pty Ltd | Victoria |
| Equilibrium Interactive Pty Ltd | |
| Equipoise (International) Pty Ltd | Queensland |
| ERA Communications | |
| Era Media | |
| Erico IT Services | |
| Eritheia Labs | |
| Erudite Technologies | New South Wales |
| ESB Consultancy | Western Australia |
| EscapeNet | South Australia |
| eSkilled AI Course Creator | |
| eSolutions | New South Wales |
| Esoteric Technologies | Victoria |
| Espay Pty Ltd | New South Wales |
| Esper Satellite Imagery | |
| Esperance Communications | Western Australia |
| Espresso Apps Pty Ltd | |
| ESRI Australia Pty Ltd | Queensland |
| eStrategies Pty Ltd | New South Wales |
| ETech Computers | |
| etech Consulting | |
| ETM Pacific Pty Ltd | New South Wales |
| Etraffic Group Pty Ltd | |
| Eucalyptus | New South Wales |
| EUX Digital Agency | New South Wales |
| Evans Electric | New South Wales |