| BioScience Managers Pty Ltd | |
| Biotron Ltd | New South Wales |
| BirdBrain | Western Australia |
| Bissano Software | Queensland |
| Bit Loud Digital | Victoria |
| Bitplex Pty Ltd | |
| BitScope | New South Wales |
| Bitten by Design | New South Wales |
| Biza Pty Ltd | Queensland |
| BizCatalyst | Victoria |
| Bizpep | Queensland |
| Biztactix | Victoria |
| Bizz Wizz Software | Western Australia |
| BJ Communications | Victoria |
| BJ2DESIGN | New South Wales |
| Bjango Pty Ltd | Victoria |
| BJM Digital | |
| Black Sails | |
| Black Shark Digital | |
| Black Sheep Capital | Queensland |
| Blackbird Technology | |
| Blackbird Ventures | New South Wales |
| Blackbook Artificial Intelligence | Queensland |
| BlackCompass Software Pty Ltd | |
| Blacklight Productions Pty Ltd | New South Wales |
| Blackmagic Design Pty Ltd | |
| Blake Digital | Western Australia |
| Blank Page Software Solutions | |
| Blaq | New South Wales |
| Blend AI | |
| Blink Creative | |
| Blinq Technologies Pty Ltd | |
| Blis Web Agency | South Australia |
| Bliss Media | Victoria |
| Blitz Marketing | |
| Blitzo Studio | |
| Block Earner | New South Wales |
| Blockchain Australia | Victoria |
| Blockchain Technologies | New South Wales |
| Blockstars Technology Pty Ltd | |
| BlockTexx | |
| Bloocow | Western Australia |
| Bloom Digital | Western Australia |
| BloomIT Consulting Pty Ltd | New South Wales |
| Bloomtools | Queensland |
| BluCloud | |
| Blue Cherry | Western Australia |
| Blue Crystal Solutions | South Australia |
| Blue Line Software Pty Ltd | New South Wales |
| Blue Poppy Ventures | South Australia |