| Interstudio S.r.l. | Pistoia |
| Intertraff | Taranto |
| Intesa S.r.l. | Como |
| Intesa Sanpaolo | Turin |
| Intesa Sanpaolo Innovation Center S.p.A. | |
| Intesys S.r.l. | Verona |
| Intrauma S.p.A. | |
| Intred | Brescia |
| Inventio.ai | |
| Inverse Path | Friuli-Venezia Giulia |
| Invest in Lombardy | Milan |
| Involve | |
| INVRSION S.r.l. | |
| Inweb Adriatico S.r.l. | Pescara |
| Inxpect S.p.A. | |
| InYourLife S.r.l. | Florence |
| Iottly | |
| iPhoneAssistRoma | Rome |
| iPhoneHospital | Turin |
| IPKom S.r.l. | Arezzo |
| IPnext S.r.l. | Milan |
| IppocraTech | |
| Iprogrammatori S.r.l. | Milan |
| IPSA S.r.l. | |
| Ipses S.r.l. | Milan |
| Ipsnet | Turin |
| IPTSAT S.r.l. | |
| iQuad | Varese |
| IRBM S.p.A. | |
| IRCE S.p.A. | Bologna |
| IRETH | Turin |
| IRIDEOS S.p.A. | Milan |
| Irion S.b.r.l. | |
| IrisNet Solutions | Pisa |
| iRoma S.r.l. | |
| Irpinia Linux User Groups | Avellino |
| Irriworks S.r.l. | Palermo |
| IRS International Recovery Service | Friuli-Venezia Giulia |
| ISAAC S.r.l. | |
| ISAC S.r.l. | |
| Ischia Web Software | Naples |
| ISI Foundation | Turin |
| Isicom S.r.l. | Modena |
| Isiweb S.r.l. | Monza and Brianza |
| Iso Data S.r.l. | |
| Iso Sistemi S.r.l. | Genoa |
| iStarter S.p.A. | |
| Istella S.p.A. | |
| Istella S.r.l. | Cagliari |
| Istituto di Ricerche Farmacologiche Mario Negri | |