| up2date Software | Baden-Württemberg |
| Up42 GmbH | |
| upCast | Bavaria |
| Upcyte Technologies | Hamburg |
| Update Texware GmbH | Bavaria |
| UpdateStar | Berlin |
| Upgrade Hamburg | |
| UPIC GmbH | Bavaria |
| upjers GmbH | Bavaria |
| Upline GmbH | Bavaria |
| UPLINK Network GmbH | |
| UpsellGuru GmbH | |
| Upstream Agile GmbH | Berlin |
| Uptime Informations-Technologie GmbH | Hamburg |
| Upvest GmbH | |
| UpVisit | |
| Urania Ventures GmbH | |
| URBAN Tetrasys GmbH & Co. KG | Baden-Württemberg |
| Urbane Daten-Systeme GmbH | Hamburg |
| Urbanistic GmbH | |
| Urbanstudio GmbH | |
| Urbify Technologies GmbH | |
| URL-Services e.K. | Hesse |
| urlscan GmbH | |
| Uroquant | |
| UroQuant Diagnostics GmbH | |
| Usability & UX SEO Blog | Berlin |
| Usability Testessen | Hesse |
| usb Gesellschaft für Unternehmensberatung und Systementwicklung mbH | Bavaria |
| USDX Wallet | |
| Usedom Design | Mecklenburg-Vorpommern |
| usedSoft Deutschland GmbH | North Rhine-Westphalia |
| Usegroup | Hesse |
| USENEXT | Bavaria |
| User Interface Design GmbH | Baden-Württemberg |
| Usercentrics GmbH | |
| Userlane GmbH | Bavaria |
| Userlike UG (haftungsbeschränkt) | North Rhine-Westphalia |
| USM | Bavaria |
| UST GmbH | Baden-Württemberg |
| USU Software AG | Baden-Württemberg |
| Ute & Gerhard Weber | Bavaria |
| Utilitas GmbH | North Rhine-Westphalia |
| Utimaco GmbH | |
| Utomi GmbH | Bavaria |
| Utpatti GmbH | |
| UVALASoft | |
| Uwe Electronic GmbH | Bavaria |
| UWebbing Internet Services | North Rhine-Westphalia |
| UX3D GmbH | |