| Ulrike Stehling | North Rhine-Westphalia |
| UltiHash GmbH | |
| Ultimate | |
| ultimate.ai | Berlin |
| Ultra Consulting Network GmbH | North Rhine-Westphalia |
| Ultra Tendency | |
| Ultracolor | Berlin |
| Ultramarin GmbH | North Rhine-Westphalia |
| Ultron AG | North Rhine-Westphalia |
| Ulysses GmbH & Co. KG | Saxony |
| UM-Electronic GmbH | Baden-Württemberg |
| UMA Electronic Productions GmbH | Bavaria |
| UMAN Universal Media Access Networks GmbH | Baden-Württemberg |
| UMEC elektronische Komponenten GmbH | Baden-Württemberg |
| Umesoft GmbH | |
| UMGIS Informatik GmbH | Hesse |
| Umlaut.bio GmbH | |
| UMT United Mobility Technology AG | |
| Umwelt-Campus Birkenfeld | |
| Umweltdruck Berlin GmbH | |
| Uncap | |
| Uncap GmbH | |
| Unchained Robotics GmbH | |
| Unclassified Software Development | Bavaria |
| uncover-it media | Hamburg |
| understand.ai | Baden-Württemberg |
| undev Studio | Berlin |
| Undpaul GmbH | Lower Saxony |
| Ungarischen Institut München e.V. | |
| Uni-App | |
| UNI:CODE IT Solutions GmbH | |
| UNIA GmbH | North Rhine-Westphalia |
| Unibox | |
| UNIBRAND GmbH | |
| Unicam Software GmbH | Bavaria |
| Unicepta GmbH | |
| Unicom Werbeagentur GmbH | Berlin |
| UNICOMP Computer Systeme GmbH | Hesse |
| Unicon GmbH | |
| Unicon Software Entwicklungs- und Vertriebsgesellschaft mbH | Baden-Württemberg |
| Unicontrol Systemtechnik GmbH | Saxony |
| UNICORE | North Rhine-Westphalia |
| Unicorn.Berlin WEM GmbH | |
| Unicorns in Tech | |
| UNIDAS Universal-Data-Systems | Hesse |
| Unidienst GmbH | Bavaria |
| Unified Automation GmbH | |
| UNIKIMS GmbH | |
| Unikraft GmbH | |
| Unilab AG | North Rhine-Westphalia |