| Acounting & Computer Solutions, Inc. | District of Columbia |
| Acoustic Web Design | Louisiana |
| AcousticaBio | |
| Acperion | Colorado |
| Acpharis | Massachusetts |
| ACprint, Inc. | Texas |
| Acqueon Technologies, Inc. | Texas |
| Acquia, Inc. | Massachusetts |
| AcquiData, Inc. | Florida |
| Acquire | California |
| Acquire App | California |
| Acquire Digital | |
| Acquire Internet Marketing | |
| Acquire.com, Inc. | California |
| Acquired Data Solutions, Inc. | Maryland |
| ACR Electronics, Inc. | Florida |
| ACRC Trials | Texas |
| Acre Venture Partners | |
| Acrhonix Semiconductor Corporation | California |
| ACRi | California |
| Acrigen Biosciences | California |
| Acritum Software | California |
| Acrivon Therapeutics | |
| Acro Biotech, Inc. | California |
| Acro Software, Inc. | Virginia |
| Acro Systems, Inc. | Maryland |
| Acrobat Genomics | California |
| Acrobatic Cow | |
| Acromag | Michigan |
| Acroname | Colorado |
| Acronis SCS | Arizona |
| Acronotics, Inc. | |
| Acronova Technology, Inc. | New Jersey |
| Acronym | |
| Acronym Media, Inc. | New York |
| Acronym Venture Capital | New York |
| Acrosonic | Washington |
| Across Communications, Inc. | Minnesota |
| Across Platforms Television Advertising and Analytics, Inc. | Massachusetts |
| Acrosync, LLC | |
| Acrosys Corporation | Texas |
| Acrotech Biopharma, Inc. | |
| AcroVista, LLC | Texas |
| Acryl Data | California |
| ACS Business Systems, Inc. | Colorado |
| ACS Data Recovery | Texas |
| ACS Diagnostics | California |
| ACS Digital | California |
| ACS Industrial Services, Inc. | Maryland |
| ACS Quantum Design | Washington |