| Aplicaciones Electrónicas Quasar, S.A. | Madrid |
| Aplicaciones en Informática Avanzada, S.L. | Barcelona |
| Aplicaciones.AI | |
| Aplitec Informàtica, S.L. | Girona |
| App&Web | Granada |
| AppAquí | Barcelona |
| Appcinking | |
| AppFutura | Barcelona |
| Appglobal | Madrid |
| AppGround | Santa Cruz de Tenerife |
| Apple La Maquinista | Barcelona |
| Apple Parquesur | Madrid |
| Apple Xanadú | Madrid |
| Applied Research using OMIC Sciences, S.L. | |
| Applivery, S.L. | Madrid |
| Applus+ | |
| Appnormals Team, S.L. | Barcelona |
| apps4two | Valencia |
| Appylan Solutions | Madrid |
| Apralize Innovations | |
| ApSIC, S.L. | Barcelona |
| APSL Tech | Balearic Islands |
| AptaTargets, S.L. | Madrid |
| Aptus Biotech | Madrid |
| Aqua eSolutions | Madrid |
| Aquora Business Education, S.L. | |
| Araclon Biotech | Zaragoza |
| Araelec, S.L. | Zaragoza |
| Aragonese Foundation for Research & Development | |
| Aral Roca | |
| ARATECH Lifestyle Technology, S.L. | Madrid |
| Arbol Computer | Barcelona |
| Arcadina | Valencia |
| Architechtures | Málaga |
| Archivel Farma, S.L. | Barcelona |
| Arditec Sistemas | Barcelona |
| Arditech Nuevas Tecnologias | Valencia |
| AREA Software Solutions | Barcelona |
| Área Web Creativa | Castellón |
| Area25 | Granada |
| Arena Financial Tech | Madrid |
| Arenet | Madrid |
| Arengu | A Coruña |
| ARG Consulting | Murcia |
| Argilla | Madrid |
| Argus Disseny | Barcelona |
| ARION Grupo | Sevilla |
| Aritmos | Lleida |
| Arkadel 21, S.L. | Madrid |
| Arkadia Space | Castellón |