| Bioingenium | Barcelona |
| Biointaxis, S.L. | |
| BIOMAR Microbial Technologies | León |
| Biomedal, S.L. | Sevilla |
| Bioo | Barcelona |
| Biorce | |
| BioSim | Madrid |
| Bit2Me | Valencia |
| Bitban Technologies, S.L. | Madrid |
| Bitel Group | Valladolid |
| Bitergia | |
| BITmarketing | |
| BITmarketing Online SLNE | Alicante |
| BitMetrics | Barcelona |
| Bitnis.com | Pontevedra |
| Bitnova Identificación y Control, S.L. | Murcia |
| Bitnovo | Valencia |
| Bits | Madrid |
| Bittacora Informática y Diseño, S.L. | Badajoz |
| Bittopia.com | Madrid |
| Bizkaia Formación | Biscay |
| Black Elk Studios | Málaga |
| Blackhills Diagnostic Resources, S.L.U. | |
| Blackpearl Capital Management SGEIC, S.A. | |
| Blamar Automatización y control, S.A. | Madrid |
| Blanquerna - Universitat Ramon Llull | |
| Blast Off Partners, S.L. | Castellón |
| Bloc d'apple en català | Barcelona |
| Blockchers | Navarre |
| Blogalia | Las Palmas |
| Blogestudio, S.L. | Valencia |
| BlogsterApp Ambassador, S.L. | Madrid |
| Bloobirds | Barcelona |
| Bloomium | Barcelona |
| Blueant Solutions | |
| BlueFloat Energy International, S.L.U. | Madrid |
| Bnext | Madrid |
| BNP Paribas, S.A. | |
| Bocetos | Madrid |
| Bold Valuable Technology Spain, S.L. | Barcelona |
| Bonet-Galiano-Angulo S.I. S.L. | Valencia |
| Bonsai Partners | Madrid |
| Bonsai Venture Capital | Madrid |
| Bookline Solutions, S.L. | |
| Borah Digital Labs, S.L. | |
| Borax | Barcelona |
| Borneo Analytics | Gipuzkoa |
| Boston Tech, S.L. | Madrid |
| Botigues.cat | Girona |
| Botslovers | |