| Sitni Sati | City of Zagreb |
| SL solucije d.o.o. | |
| Slicejack | Split-Dalmatia |
| Smart Work j.d.o.o. | |
| SMBWireless | City of Zagreb |
| SMD Micro-elektronika | City of Zagreb |
| Sofnet d.o.o. | City of Zagreb |
| SoftPro Tetral | City of Zagreb |
| Software Sauna d.o.o. | |
| SoftWare.HR | |
| Softwise d.o.o. | Međimurje |
| Šokčić d.o.o. | Osijek-Baranja |
| Soltech | |
| Solucija | City of Zagreb |
| Šon produkt d.o.o. | Koprivnica-Križevci |
| South Central Ventures | City of Zagreb |
| Span d.o.o. | City of Zagreb |
| Sparky Solution d.o.o. | City of Zagreb |
| Speck d.o.o. | |
| Spectral Core Ltd | Istria |
| SPECTRE d.o.o. | |
| Sphera Project Development & Design | Split-Dalmatia |
| Spin d.o.o. | Osijek-Baranja |
| SpinMedia Development Studio | Primorje-Gorski Kotar |
| Split Tech City | Split-Dalmatia |
| Spring Media | |
| Staroslavenski institut | |
| Start Digital | |
| Startup Incubator Rijeka | |
| Status d.o.o. | Primorje-Gorski Kotar |
| Stemi Education | |
| STORM Computers d.o.o. | City of Zagreb |
| Strongetic | |
| Studentski Poduzetnički Inkubator | |
| STUDIO 33 d.o.o. | |
| Studio elektronike Rijeka d.o.o. | Primorje-Gorski Kotar |
| Studio HERON d.o.o. | Brod-Posavina |
| Studio HS internet d.o.o. | Osijek-Baranja |
| Studio Revolucija | City of Zagreb |
| Studio Revolucija | |
| Studio4web | Krapina-Zagorje |
| Subpixel d.o.o. | |
| SUBPIXEL d.o.o. | |
| Superius | |
| SuperNet d.o.o. | City of Zagreb |
| Supra Net d.o.o. | |
| Sustinere | |
| SV Group d.o.o. | City of Zagreb |
| Sve je dobro j.d.o.o. | Zagreb |
| Sveučilišta u Dubrovniku | Dubrovnik-Neretva |