| Two Falls | |
| Two Hat Security | British Columbia |
| Two Potato Ltd | Ontario |
| Two Small Fish Ventures | |
| Two Story Robot | British Columbia |
| Two Stroke Lead, Inc. | British Columbia |
| Two Tall Totems Ltd | |
| Two Tall Totems Ltd | |
| Two-Photon Research Canada, Inc. | |
| Twotinydice | |
| TX/communications Canada, Inc. | Ontario |
| TXT E Solutions, Inc. | |
| TxtSquad Enterprise, Inc. | |
| Tydra Laboratories | |
| Tygus | Alberta |
| Tyltgo | |
| Tyndale University | Ontario |
| Tyne Engineering | Ontario |
| TYPE5 | |
| Typhoon IT | Ontario |
| Tyto Robotics, Inc. | |
| U Innovations | Quebec |
| U15 Group of Canadian Research Universities | Ontario |
| U2 Ventures | |
| U2GO | |
| U7 Solutions | Ontario |
| U92 | Quebec |
| UBC BizTech | |
| UBC Orbit Satellite Design Team | |
| UBC Sauder School of Business | British Columbia |
| Ubenwa Health | |
| Ubéo | Quebec |
| UBERbase | British Columbia |
| Uberflip | Ontario |
| Ubertor | |
| UBIK VoIP | Quebec |
| Ubika Research | Ontario |
| Ubineer | Ontario |
| Ubisoft Halifax | Nova Scotia |
| Ubisoft Montréal | Quebec |
| Ubisoft Saguenay | |
| Ubisoft Sherbrooke | Quebec |
| Ubisoft Toronto | Ontario |
| Ubisoft Winnipeg | |
| Ubiweb | |
| UBQT | Quebec |
| uBriGene | |
| Ubuntu Québec | Quebec |
| Ucentrik | Ontario |
| Ucora | British Columbia |