| Translucent Computing, Inc. | Ontario |
| Transmitter Studios | Ontario |
| Transoft Solutions | |
| Transparent Solutions | British Columbia |
| Transpera Technologies, Inc. | Alberta |
| Transpire Technologies | |
| TransPod | Ontario |
| Transpolar Technology Corporation | Ontario |
| Transtech Innovations | |
| TransUnion of Canada, Inc. | |
| Tranthom Informatique | Quebec |
| Tranzeo Wireless | British Columbia |
| Trapeze Group | Ontario |
| Travancore Analytics | |
| TravelCurve, Inc. | Ontario |
| Travis Software, Inc. | |
| TravPRO Technologies | Alberta |
| Trawna Publications | Ontario |
| Traxxall Technologies | |
| Traysoft | Ontario |
| Tread | |
| Treadwell Therapeutics | |
| Treasure Web Designs | British Columbia |
| TreasureStone, Inc. | Alberta |
| TREAT | Ontario |
| Trebas Institute Québec, Inc. | |
| Trebuchet | Quebec |
| Treefog | Ontario |
| Treefort Technologies, Inc. | |
| Treefrog Accelerator | |
| Treefrog Digital | |
| Treehaus Collaborative Workspace | |
| Treeline Capital | |
| Treize | |
| Trek Marketing | |
| Trek PC Computers, Inc. | British Columbia |
| Trek-IT, Inc. | Ontario |
| Tremain Media | British Columbia |
| Tremar Computer Solutions | Alberta |
| Tremb Services Ltd | British Columbia |
| Tremor Technology Group, Inc. | Ontario |
| Trend Research, Inc. | |
| Trendex Information Systems, Inc. | Quebec |
| Trendigo | |
| TrendMetrix Software, Inc. | Ontario |
| Trends Electronics International, Inc. | British Columbia |
| Trent University | Ontario |
| Trent University - Computer Science | Ontario |
| Trent University - Mathematics | Ontario |
| Tresseo | |