| DiscoveryLab | |
| Dish Productions | Ontario |
| Dishout IT Solutions | Ontario |
| Dispersa | |
| Dispersa, Inc. | |
| Displaid | |
| Display Systems International | Saskatchewan |
| Disruption Ventures | Ontario |
| Disruptive Publishers | |
| Distancia | Quebec |
| DistantJob | Quebec |
| Distech Controls | Quebec |
| Distility | |
| DistillerSR, Inc. | |
| Distinct Capital Partners, Inc. | |
| Distinct SEO | Alberta |
| Distributed Computing Experts Corporation | Alberta |
| Distributel | Quebec |
| District 7 Digital | Ontario |
| District M | Quebec |
| District Ventures Capital | |
| DistriQ | Quebec |
| DistroWatch.com | British Columbia |
| Disus, Inc. | Ontario |
| DIT Web Solutions, Inc. | Ontario |
| Ditch Labs | |
| Dittybase Technologies, Inc. | British Columbia |
| Diva Communications | |
| Divcom Software | British Columbia |
| Divelink Underwater Communications | British Columbia |
| Diverse Electronics | Quebec |
| Diverse Marketing | |
| Divestco | Alberta |
| Divi Diva | Ontario |
| Divise Telecom Solutions | Ontario |
| Division 3 Communications, Inc. | Ontario |
| DivNotes, Inc. | Ontario |
| DIVORCEmate Software, Inc. | Ontario |
| Dix-Onze, Inc. | Quebec |
| DIY Robotics | |
| DJ Communications | Alberta |
| DJH Training & Application Solutions, Inc. | Ontario |
| DL Custom | Ontario |
| DLS Technology Corporation | Ontario |
| DMA Digital Media Group, Inc. | British Columbia |
| DMC Group | |
| DMD Marketing, Inc. | Ontario |
| DMG Blockchain Solutions | British Columbia |
| DMIB, Inc. | Quebec |
| DMS Consulting | Quebec |